FACScan,FC500, LSRII Policies and Procedures

  • Only previously trained researchers are allowed to operate the analyzers.
  • You are billed according to time booked. Therefore, please book time accordingly. When your time is over, either stop acquisition or book more time if needed and available. Discrepancies can and will be monitored.
  • When booking time, each researcher is responsible to provide the following information: Full name (no nicknames), phone number, PI, and grant number.
  • Each individual user is responsible for the proper maintenance of the analyzers. This includes, but not limited to, filling the sheath tank, emptying the waste tank, flushing the sample lines with 10% bleach followed by dH2O, and cleaning the sample probe after each use (protocols will be included). Please leave the instruments in standby with 1ml. of dH20 for the next scheduled individual.
  • The first scheduled appointment on any given day is responsible for properly starting up the instruments. This includes allowing for the optimal 30 minute laser warm up and proper priming of the fluidics.
  • The last scheduled appointment on any given day is responsible for properly shutting down the instruments. This includes leaving the analyzers in standby mode, before turning off power, with 1ml of dH2O on the sample port. If there is any doubt about last scheduled appointment, then please follow the 4PM rule.
  • The 4PM rule is as follows: if using the analyzers after 4PM and you are unsure (for any reason) if someone is using the analyzers after you, please SHUT IT DOWN!!!
  • Bleach, dH2O, sheath fluid, biohazard containers, paper, and trash are provided by the core facility. Each user is responsible for providing their own tubes, data storage materials, racks, pipettes, tips, and gloves. If you notice supplies running low, please let us know so we can replenish them.
  • Gloves MUST be worn when using the cytometers.
  • Each user is responsible for their own data management. This means exporting and saving data properly and deleting it from the drive. Any data left longer than a month will be deleted by the facility and user charged for the time.
  • Please consider the acknowledgement of these policies as a first warning. Subsequent compliance failures will be handled as follows: First offense, meeting with the flow facility to be re-trained on proper instrument usage. Second offense, meeting with flow facility and your PI to discuss proper instrument usage. Third offense, loss of privileges.

Daily Fluidics Cleaning

After each use, clean the sample injection tube and the area between the injection tube and the outer sleeve. This prevents the injection tube from becoming clogged and removes dyes that can remain in the tubing.

FACScan and LSRII's
  1. Move the tube support arm to the side.
  2. Install a tube containing 3ml. of 10% bleach on the SIP. Allow the vacuum to aspirate for approximately 1 minute.
  3. Press the HI sample flow rate button and place the tube support arm under the tube. Allow the bleach to run for 5 minutes.
  4. With the tube support to the side, install a tube with 3ml. of dH2O. Allow the vacuum to aspirate for approximately 1 minute.
  5. With flow rate remaining on HI, place the tube support arm under the tube. Allow the dH2O to run for 5 minutes.
  6. Leave a tube containing 1ml. of dH2O on the SIP with the support arm under the tube. Note: do not leave more than 1ml. of dH2O on the SIP.
  1. Put test tube of 10% bleach into carousel position 1.
  2. Put 3 tubes of distilled H20 into the carousel positions 2,3, and 4.
  3. Select cleanse. PNL file from panels list.
  4. Proceed as if running samples.

Daily Shutdown

FACScan and LSRII's
  1. Set the cytometer to STNDBY mode.
  2. Turn off the flow cytometer.

Double click Fcoff icon on desktop.