FACScan,FC500, LSRII Policies and Procedures
Only previously trained researchers are allowed to operate the
You are billed according to time booked. Therefore, please book time
accordingly. When your time is over, either stop acquisition or book
more time if needed and available. Discrepancies can and will be
When booking time, each researcher is responsible to provide the
following information: Full name (no nicknames), phone number, PI, and
Each individual user is responsible for the proper maintenance of the
analyzers. This includes, but not limited to, filling the sheath tank,
emptying the waste tank, flushing the sample lines with 10% bleach
followed by dH2O, and cleaning the sample probe after each use
(protocols will be included). Please leave the instruments in standby
with 1ml. of dH20 for the next scheduled individual.
NEVER EVER LEAVE THE MACHINE IN RUN!!!!!!!!
The first scheduled appointment on any given day is responsible for
properly starting up the instruments. This includes allowing for the
optimal 30 minute laser warm up and proper priming of the fluidics.
The last scheduled appointment on any given day is responsible for
properly shutting down the instruments. This includes leaving the
analyzers in standby mode, before turning off power, with 1ml of dH2O
on the sample port. If there is any doubt about last scheduled
appointment, then please follow the 4PM rule.
The 4PM rule is as follows: if using the analyzers after 4PM and you
are unsure (for any reason) if someone is using the analyzers after
you, please SHUT IT DOWN!!!
Bleach, dH2O, sheath fluid, biohazard containers, paper, and trash are
provided by the core facility. Each user is responsible for providing
their own tubes, data storage materials, racks, pipettes, tips, and
gloves. If you notice supplies running low, please let us know so we
can replenish them.
Gloves MUST be worn when using the cytometers.
Each user is responsible for their own data management. This means
exporting and saving data properly and deleting it from the drive. Any
data left longer than a month will be deleted by the facility and user
charged for the time.
Please consider the acknowledgement of these policies as a first
warning. Subsequent compliance failures will be handled as follows:
First offense, meeting with the flow facility to be re-trained on
proper instrument usage. Second offense, meeting with flow facility and
your PI to discuss proper instrument usage. Third offense, loss of
Daily Fluidics Cleaning
After each use, clean the sample injection tube and the area between the
injection tube and the outer sleeve. This prevents the injection tube from
becoming clogged and removes dyes that can remain in the tubing.
FACScan and LSRII's
Move the tube support arm to the side.
Install a tube containing 3ml. of 10% bleach on the SIP. Allow the
vacuum to aspirate for approximately 1 minute.
Press the HI sample flow rate button and place the tube support arm
under the tube. Allow the bleach to run for 5 minutes.
With the tube support to the side, install a tube with 3ml. of dH2O.
Allow the vacuum to aspirate for approximately 1 minute.
With flow rate remaining on HI, place the tube support arm under the
tube. Allow the dH2O to run for 5 minutes.
Leave a tube containing 1ml. of dH2O on the SIP with the support arm
under the tube. Note: do not leave more than 1ml. of dH2O on the SIP.
Put test tube of 10% bleach into carousel position 1.
Put 3 tubes of distilled H20 into the carousel positions 2,3, and 4.
Select cleanse. PNL file from panels list.
Proceed as if running samples.
FACScan and LSRII's
Set the cytometer to STNDBY mode.
Turn off the flow cytometer.
Double click Fcoff icon on desktop.