Fluorescent Activated Cell Sorters

Beckman Coulter MoFlo AstriosEQ 4 laser system: A high speed cell sorter (up to 70,000 cells/second) and analyzer (up to 100,000 cells/second), with four fixed lasers (405nm, 488nm, 561nm, and 640nm). The AstriosEQ is capable of acquiring 28 parameters simultaneously. In addition, it contains two forward scatter parameters and three side scatter parameters, allowing for analysis of extracellular vesicles. Two-, Four-, and Six-way sorting is available. Sorting can be done into tubes of various sizes, plates and custom slides. Single cell sorting into plates is also available.

Operating software for the MoFlo AstriosEQ is the Beckman Coulter Summit program, version 6.3. Operation of this instrument requires a dedicated technician to align lasers, maintain the instrument and to assure its reliable and effective utilization.

Beckman Coulter MoFlo AstriosEQ 7 laser system: A high speed cell sorter (up to 70,000 cells/second) and analyzer (up to 100,000 cells/second), with six fixed lasers (405nm, 488nm, 532nm, 561nm, 592nm, and 640nm) and one spatially separated UV (355nm). The AstriosEQ is capable of acquiring 32 parameters simultaneously. In addition, it contains two forward scatter parameters and five side scatter parameters, allowing for analysis of extracellular vesicles. Two-, Four-, and Six-way sorting is available. Sorting can be done into tubes of various sizes, plates and custom slides. Single cell sorting into plates is also available.

Operating software for the MoFlo AstriosEQ is the Beckman Coulter Summit program, version 6.3. Operation of this instrument requires a dedicated technician to align lasers, maintain the instrument and to assure its reliable and effective utilization.

Becton Dickinson SORP FACSAria II: A special order five laser cell sorters (up to 22,000 cells/second) and analyzer (up to 70,000 cells/second) equipped with a solid state 50mW 488nm laser, JDS Uniphase 40mW 640nm HeNe laser, Coherent Compass 40mW 561nm laser (allowing for the acquisition, analysis and sorting of a variety of fluorescent proteins), Lightwave Mode Lock 60mW 355nm UV laser, and Coherent VioFlame 50mW 405nm violet laser. Besides the forward and side scatter parameters, the instrument is equipped for detection of four fluorescent parameters from the 488nm laser, three parameters from the 640nm laser, five parameters from the 561nm laser, three parameters from the violet laser and three parameters from the UV laser. Sorting can be done into tubes of various sizes, plates and custom slides. Single cell sorting into plates is also available.

Operating software for the FACSAria II is the Becton Dickinson Diva software, version 6.11, operating on an HP xw4600 Core 2 duo. Operation of this instrument requires a dedicated technician to maintain the instrument and to assure its reliable and effective utilization.

Propel Avalon/S3: The Avalon Cell Sorter is the first truly walk-away automated cell sorter. Real-time monitoring and smart features make cell sorting easier and accessible to both novices and experts. The Avalon is equipped with a 488nm Blue laser and 561nm Yellow laser. Sorting is done at low pressure utilizing a 100um nozzle tip.

  • Hands-free nozzle tip alignment — features the fully automatic AutoGimbal™ System, driven completely by software
  • Simplified instrument setup — fully automated drop delay calculation, stream alignment, and droplet break-off monitoring enable precise 1–4 color sorting with minimal training

Operating software for the Avalon is the BioRadProSort program, version 4.3, running on a Dell Precision Intel Core 2 duo. Operation of this instrument requires training before usage.

Flow Cytometry Analyzers

Becton Dickinson SORP LSR II: This is a special order five laser, benchtop analyzer equipped with a solid state 50mw 488nm laser, JDS Uniphase 40mW 640nm laser, a Coherent Compass 25mW 561nm laser (allowing for the acquisition and analysis of a variety of fluorescent proteins), a Coherent VioFlame 50mW 405nm laser, and a Mode-Lock 20mW 355nm laser. This allows for detection of four fluorescent parameters from the 488nm laser, three parameters from the 640nm laser, five parameters from the 561nm laser, three parameters from the violet laser and three parameters from the UV laser. Also, the instrument is equipped with an HTS (High Throughput sampler) for acquisition of 96, 384, and other customizable plate sizes. Operating software for the LSR II is the Becton Dickinson Diva software, version 6.11, operating on a HP xw4600 Core 2 duo. Individual research personnel, following requisite training from the Flow Cytometry Core staff, may independently use and operate this instrument. It is available to trained users for full use even in off hours, including evenings, nights, and weekends. Access to the instrument during off hours is controlled by a coded electronic card access system, which limits access to approved users only. (Please note that support for this instrument will be ending in December 2019.)

Two Beckman Coulter Gallios: The Gallios is a three laser benchtop analyzer equipped with a 22mW Argon 488nm laser, 25mW 638 laser, and a 40mW 405nm laser. All are solid state diodes which are spatially separated at 125um. Alignment is software controlled using Cyto System Mgmt software and can be controlled remotely. This allows for detection of five fluorescent parameters from the 488nm laser, three parameters from the 638nm laser, and two parameters from the 405nm laser in a proprietary "Boulevard" configuration for greater fluorescent resolution. With multiple measurements and angles of Forward Scatter and a gel coupled pickup filter lens assembly, the Gallios can resolve 0.4um diameter particles from background and maximize detection up to 40um. This instrument is equipped with a Multi-tube Carousel Loader system to automatically analyze multiple samples. Software for acquisition and analysis is the Beckman Coulter CXP, running on an Intel Pentium Core 2 Duo processor. Individual research personnel, following requisite training from the Flow Cytometry Core staff, may independently use and operate this instrument. It is available to trained users for full use even in off hours, including evenings, nights, and weekends. Access to the instrument during off hours is controlled by a coded electronic card access system, which limits access to approved users only. (Please note that support for this instrument will be ending in December 2020.)

Cyntellect Celigo: The Celigo adherent cell cytometer allows in situ brightfield and fluorescent analysis of adherent cells. Cell number, density, area density, diameter, area, and perimeter can all be measured. Using LEDs, the system can obtain data for three fluorescent channels (ex 377nm/em 447nm, ex 483nm/em 536nm, and ex 531nm/em 629nm) and 1 brightfield channel. 384, 96, 24, 12, 6, and 1536 well plates can be analyzed; as well as, T-25 and T-75 flasks. Software for acquisition and analysis is the Cyntellect Celigo software version 1.3, running on a Dell Precision T3500. Individual research personnel, following requisite training from the Flow Cytometry Core staff, may independently use and operate this instrument. It is available to trained users for full use even in off hours, including evenings, nights, and weekends. Access to the instrument during off hours is controlled by a coded electronic card access system, which limits access to approved users only.

Two Beckman Coulter CytoFLEX LX: The instrument has the capacity for 23 parameters, including 21 for fluorescence detection. The fully activated instrument includes three fluorescent channels from the 355 nm (UV) laser, five from the 405 nm (Violet) laser, three from the 488 nm (Blue) laser, five from the 561 nm (Yellow Green) laser, three from the 638 nm (Red) laser, and two from the 808 nm (Infrared) laser. Option to configure Avalanche Photo Diode detector array to collect side scatter signal from Violet (405 nm) laser. The configured channel (VSSC) can be used to better resolve nanoparticles. 96-well Standard Flat, U and V bottom plates, and 96-deep well plates.

Operating software for the CytoFLEX LX is the Beckman Coulter CytExpert program, version 2.3. Operation of this instrument requires training by the Flow Core faculty. CytExpert software is available for free download.

Izon Science qNano: The qNano Gold measures particles using the Tunable Resistive Pulse Sensing (TRPS) principle. TRPS is the most powerful particle measurement system available for nano and sub-micron particle (40nm-10um) measurement and analysis. The qNano Gold instrument suite is therefore an extremely powerful and accurate tool, superseding old laser based ensemble methods. It measures individual particles with the highest accuracy and repeatability available for, particle size and real distribution, concentration and surface charge.

Analysis Software

FlowJo: FlowJo software reads flow cytometry data and facilitates complex data analysis in a graphically intuitive way.

Beckman Coulter Kaluza: Kaluza Software challenges today´s solutions with a new vision of what flow cytometry analysis should be. Kaluza provides visualization tools, speed and an innovative simplicity to the flow community. With features designed to make your life easier in the lab, Kaluza aims to provide a greater understanding of the data generated by modern cytometers. Kaluza employs patent pending technology that allows for real time analysis of high content files allowing you to spend your valuable time on discovery.

CytExpert: Software for the Beckman Coulter CytoFlex data only. CytExpert software is available for a free download.

Training

Training is available for all Flow Cytometry Analyzers and the FACSAria II Cell Sorter. Trainings are provided by an experienced staff member several times monthly. Also, a "Basics of Flow Cytometry" course is offered 1-2 times per year based on request. In addition, researchers can request Facility run or Facility assisted analysis of samples.

Educational seminars and workshops are provided several times per year for the advancement of the research community.