Electron Microscopy (EM) Core
Provides Services in Electron Microscopy
The EM Core at BIDMC provides service, technical assistance, and instrumentation for electron microscopy techniques.
- Tissue processing for Transmission Electron Microscopy
- Sectioning for Transmission Electron Microscopy
- Ultrastructural analysis and image processing
Work is done on a fee-for-service basis with a
graded fee structure.
Priority use is given to BIDMC researchers and priority use and reduced fees are provided to investigators of the Harvard Digestive Diseases Center (due to the NIH-funded Digestive Diseases Center grant).
Equipment in the facility includes:
JEOL 1400 (photo, right)
- Installed January 2010 and is state-of-the-art.
- High contrast
- Gatan Orius 11 MegaPixel CCD Cameras for photodocumentation
- SerialEM, IMOD, and Chimera EM Tomography software.
- Leica Ultracut E Ultramicrotome
- Leica Ultracut S Cryo-Ultramicrotome
- Leica glass knife breaker
- Baltec-Med 020 Vacuum Evaporator
- Wohlwend Compact 02 High Pressure Freezer
The JEOL1400 transmission electron microscope and Wohlwend HPF are available for independent use by any researcher at the BIDMC who receives training by core staff.