Non-Coding RNA Core Facility
Non-Coding RNA Precision Diagnostics and Therapeutics Core Facility
The ncRNA Core is a state-of-the-art informatics, sequencing, imaging, delivery, preclinical facility dedicated to non-coding RNA at Beth Israel Deaconess Medical Center (BIDMC) and Harvard Medical School (HMS). We will offer the following services to all researchers:
- Detection, quantification and discovery of ncRNAs including in research and clinical specimens
- Specialized bioinformatics for interpretation of ncRNA discoveries
- Precision delivery of ncRNA therapeutics to diseased tissue in vitro
The work is done on a fee-for-service basis.
The ncRNA Core is co-directed by Dr. Frank Slack, Director of the Harvard Medical School Initiative for RNA Medicine (HIRM). Established in 2014 as the Institute for RNA Medicine and hosted by the BIDMC Cancer Center, the HIRM brings together leading investigators in the field to accelerate investigations into how non-coding RNA functions and how these insights can be used to develop new therapies.
The Core will provide the following capabilities for ncRNA biology:
ncRNA Biomarker and Target Identification
- ncRNA detection via sensitive and selective qRTPCR technology from MiRXES
- statistical analyses of ncRNA detection results
Delivery of RNA and Drugs (including ncRNA)
- nanoparticle creation, packaging and delivery in vitro and in vivo using technology from Precision NanoSystems
- ncRNA downstream target and pathway analysis
In the future the Core plans to add:
- ncRNA sequencing
- ncRNA fluorescent in situ sequencing
- quantitative image analysis
- functional ncRNA characterization
Customers should contact us via email or phone with any inquiries. Requests will be reviewed and discussed with each investigator to insure appropriate services are provided. Please do not send samples without prior acceptance of a request.
If you require a quote or have any questions, please contact: Jihoon Lim.
The Delivery Unit of the ncRNA Core uses the following equipment to facilitate advances in delivery of ncRNAs to diseased tissue, and promote access to clinical trials for successful pre-clinical science:
Mini-prep system for creating custom lipid nanoparticles (LNPs) for target delivery testing.
Midi-prep device for making sufficient quantities of nanoparticles for in vitro testing.
Maxi-prep device for in vivo scale nanoparticles for delivery to model organisms, such as mouse and rat.
The Zetasizer provides both exceptionally high performance and entry level systems that incorporate combinations of a particle size analyzer, zeta potential analyzer, molecular weight analyzer, protein mobility and microrheology measurements. Particles and molecules from less than a nanometer in size to several microns can be analyzed.
Millrock Tech Freeze Dryer
This lyophilizer removes water from a product (sublimation).
The Detection Unit of the ncRNA Core uses the following equipment to facilitate quantification and idemntification of ncRNAs for novel biomarkers and targets for therapy:
- SimpliAmp Thermocyclers
- QS5-384 Well qPCR Instruments
The ncRNA Core is situated in BIDMC space ESB01A-H, which is located in the basement of the BIDMC East Building at 330 Brookline Avenue in the Longwood Medical area of Boston. Please call before visiting in person.
Hours: Mon - Fri, 9 a.m. – 5 p.m.
- Co-Director: Frank J. Slack, PhD
- Co-Director: Winston Hide, PhD
- Administrator: Jihoon Lim
- Detection Unit: TBD
- Bioinformatics Unit: TBD
- Delivery Unit: Meirav Segal, PhD