Non-Coding RNA Precision Diagnostics and Therapeutics Core Facility


The ncRNA Core is a state-of-the-art informatics/sequencing/imaging/delivery/
preclinical facility dedicated to non-coding RNA at Beth Israel Deaconess Medical Center (BIDMC) and Harvard Medical School (HMS). We will offer the following services to all researchers:

  1. Detection, quantification and discovery of ncRNAs including in research and clinical specimens
  2. Specialized bioinformatics for interpretation of ncRNA discoveries
  3. Precision delivery of ncRNA therapeutics to diseased tissue in vitro

The work is done on a fee-for-service basis.

The ncRNA Core is directed by Dr. Frank Slack, Director of the Harvard Medical School Initiative for RNA Medicine (HIRM). Established in 2014 as the Institute for RNA Medicine and hosted by the BIDMC Cancer Center, the HIRM brings together leading investigators in the field to accelerate investigations into how non-coding RNA functions and how these insights can be used to develop new therapies. Learn more about the HMS Initiative for RNA Medicine here.

Services

The Core will provide the following capabilities for ncRNA biology:

ncRNA biomarker and target identification

  • ncRNA detection via sensitive and selective qRTPCR technology from MiRXES
  • statistical analyses of ncRNA detection results

Delivery of RNA and drugs (including ncRNA)

  • nanoparticle creation, packaging and delivery in vitro and in vivo using technology from Precision NanoSystems

ncRNA bioinformatics

  • ncRNA downstream target and pathway analysis

In the future the Core plans to add:

  • ncRNA sequencing
  • ncRNA fluorescent in situ sequencing
  • quantitative image analysis
  • functional ncRNA characterization

Customers should contact us via email or phone with any inquiries. Requests will be reviewed and discussed with each investigator to insure appropriate services are provided. Please do not send samples without prior acceptance of a request.

Fees

Fee Schedule

If you require a quote or have any questions, please contact: Frank Slack at fslack@bidmc.harvard.edu

Equipment

The Delivery Unit of the ncRNA Core uses the following equipment to facilitate advances in delivery of ncRNAs to diseased tissue, and promote access to clinical trials for successful pre-clinical science:

NanoAssemblr Spark
Mini-prep system for creating custom lipid nanoparticles (LNPs) for target delivery testing.

NanoAssemblr Benchtop
Midi-prep device for making sufficient quantities of nanoparticles for in vitro testing.

NanoAssemblr Blaze
Maxi-prep device for in vivo scale nanoparticles for delivery to model organisms, such as mouse and rat.

Malvern Zetasizer
The Zetasizer provides both exceptionally high performance and entry level systems that incorporate combinations of a particle size analyzer, zeta potential analyzer, molecular weight analyzer, protein mobility and microrheology measurements. Particles and molecules from less than a nanometer in size to several microns can be analyzed.

Millrock Tech FreezeDryer
This lyophilizer removes water from a product (sublimation).

The Detection Unit of the ncRNA Core uses the following equipment to facilitate quantification and idemntification of ncRNAs for novel biomarkers and targets for therapy:

  • SimpliAmp Thermocyclers
  • QS5-384 Well qPCR Instruments
  • Contact Us

    The ncRNA Core is situated in BIDMC space ESB01A-H, which is located in the basement of the BIDMC East Building at 330 Brookline Avenue in the Longwood Medical area of Boston. Please call before visiting in person.

    Hours: Mon-Fri, 9:00 AM – 5:00 PM

    Phone: 617-735-2611

    Email: Bohyung Yoon, byoon@bidmc.harvard.edu

    Staff

    Co-Director: Frank J. Slack, PhD

    Co-Director: Winston Hide, PhD

    Administrator: Bohyung Yoon

    Detection Unit: TBD

    Bioinformatics Unit: TBD

    Delivery Unit: Meirav Segal, PhD