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a) Representative traces showing PPF of evoked MPP-GC postsynaptic currents, a measure of release probability, increases between P17 and P23 in control (WT) mice. (
b) Western blots showing expression of LGI1 and its receptor, ADAM22, between P13 and P25. (
c) Representative traces showing PPF in mature mLGI1 and mature LGI1 OE mice relative to control mice. (
d) Western blot showing expression of 64-kDa LGI1 in LGI1 OE mice and expression of 23-kDa mutant LGI1, marked by an arrow on densitometry traces, in mLGI1 mice. (
e) Quantification of 64-kDa LGI1 protein density (normalized to beta-actin) in WT, LGI1 OE and mLGI1 mice (
n = 7). (
f) Paired-pulse ratio in mature mice after treatment with the Kv1 channel blocker alpha-dendrotoxin (DTX). (
g) Quantification of paired-pulse ratio in WT, mLGI1 and LGI1 OE mice (
n = 4 to 25). *
P < 0.05; **
P < 0.01; ***
P < 0.001. Data represent means +-s.e.m.