History: The Center for Hemostasis and Thrombosis Research was founded in 1975 at Tufts University School of Medicine and New England Medical Center by Drs. Bruce and Barbara C. Furie. This laboratory has been committed to an interdisciplinary approach to the study of blood coagulation, platelet and vascular biology. The Center moved in late 1997 to its current location at Harvard Medical School within the Beth Israel Deaconess Medical Center becoming the Division of Hemostasis and Thrombosis in late 2000. The Center has a long training history in the field of blood coagulation and vascular biology; close to 100 postdoctoral and predoctoral trainees have been engaged in research in the Center.
Organization: Drs. Bruce and Barbara Furie co-direct the Furie laboratories. Senior associates within the laboratory include Dr. Alan Rigby, an NMR spectroscopist with a special interest in the determination of three dimensional protein structures and Dr. Robert Flaumenhaft , a cell biologist focused on granule secretion mechanisms in platelets. Dr. Mingdong Huang, an Xray crystallographer, joined the laboratory in September 1999. Dr. David Roth, a molecular biologist and former postdoctoral fellow in the Furie laboratory, directs an independent laboratory within the Division.
Funding: The Division for Hemostasis and Thrombosis is primarily funded by grants from the National Heart Lung and Blood Institute, National Institutes of Health. The Center is currently the recipient of a program project grant, five individual research grants, and a training grant, all from the NIH. Other support includes the Howard Hughes Medical Institute, the National Hemophilia Foundation and Burroughs-Welcome.
Core facilities: The Center for Hemostasis and Thrombosis Research maintains core facilities for modern molecular biology, protein chemistry and cell biology research. Laboratory facilities include protein sequencing, peptide synthesis, DNA synthesis, flow cytometry, computer-based molecular graphics, a leukocyte rolling video station, and fluorescence and UV spectroscopy.
The Center for Hemostasis and Thrombosis Research occupies approximately 7,500 square feet of laboratory and office space in Research East, adjacent to the Harvard Medical School quadrangle. The Center includes six
Harvard faculty members: Dr. Barbara C. Furie, Dr. Bruce Furie, Dr. David Roth, Dr. Alan Rigby and Dr. Robert Flaumenhaft ; Dr. William Aird has a secondary appointment. The Center facilities include the resources for modern biomedical research: a Becton Dickinson FACSCalibur flow cytometer, an Applied Biosystems Procise HT protein sequencer, two Applied Biosystems peptide synthesizer, an SLM 8000C spectrofluorometer with stopped flow accessory, an AVIV 118 spectrophotometer, a Varian Unity Inova 500 MHz NMR spectrometer, three Silicon Graphic computer workstations, two Sun workstations and several high end PC-based workstations.
A new confocal and widefield intravital microscopy facility for fluorescence and brightfield digital imaging has recently been completed. Miscellaneous equipment in the Center includes a Zeiss fluorescence microscope, a Milligen Cytofluor fluorescence ELISA reader, a Molecular Devices THERMOMax kinetic microELISA reader, a Coulter counter, a Pharmacia PhastSystem, high speed refrigerated centrifuges, HPLC systems, a Beckman DU6 spectrophotometer, a Packard $-scintillation spectrometer, and a Packard 5500 Autogamma spectrometer. A core transgenic facility for the preparation of mice with specific gene knockouts or transgenic mice is operated by the institution as a shared resource.
The Beth Israel Deaconess Medical Center includes modern animal facilities, a media center for the preparation of bacterial and mammalian culture media, dish washing facilities, and shared conference rooms. In addition, dark rooms, X-ray developing facilities, DNA sequencing, cell sorting, mass spectroscopy and electron microscopy are among shared resources of the institution.
The Beth Israel Deaconess Medical Center is a major biomedical research institution, and derives over $130 million per year from extramural sources, including over $80 million per year from the NIH, for its research programs. Given the adjacency of the Beth Israel Deaconess Medical Center to Harvard Medical School, Brigham & Women's Hospital, the Dana Farber Cancer Institute and Children's Hospital, the critical mass of outstanding biomedical research in a single location is of great advantage to this program. Overall, this is a unique environment for research and educational activities in the biomedical sciences.