X-ray Crystallography Core
Core Summary
The X-ray Crystallography Facility located at 99 Brookline Ave. (Research North) was recently established as a core to serve the needs of investigators in the BIDMC and Harvard Medical School community interested in using structural biology as a tool in their research.
The laboratory houses instrumentation consisting of :
- A Rigaku RUH3R rotating anode X-ray generator equipped with Osmic confocal optics
- A R-AXIS IV image plate detector
- A X-stream low temperature device for data collection at 100K
- Two Olympus microscopes are available for inspecting and photographing crystal trays
- Two incubators (4ºC - 25ºC) are available for crystallization experiments
Supporting computing facilities include several Linux PCs which can be used for data acquisition, analysis and processing.
Services
- Guidance on expression, purification and crystallization of macromolecules for X-ray crystal structure analysis
- Characterization of crystals
- Collection of X-ray data at ambient temperature and 100K
- Optimization of cryo conditions
- Data analysis and processing
- Data collection using synchrotron radiation
- Advice on phasing strategies including molecular replacement and multiwavelength methods
Personnel /Contact Information
Manager: Gabriel Birrane, PhD
Phone: 617-667 0025
Fax: 617-975 5243
Email: crystallography@bidmc.harvard.edu
