Mass Spectrometry Core
Core Summary
The Mass Spectrometry Core Facility has been serving the BIDMC community since 2004. We specialize in a targeted mass spectrometry based proteomics approach for isolated proteins and protein complexes, especially phosphorylation and other post-translational modifications involved in cellular signaling using cutting edge instrumentation. Our trained staff offers the highest quality and reliable service with meticulous attention to detail for each sample. We routinely provide services including protein identification, post-translational modification mapping, and relative peptide/protein quantification by stable isotope labeling and label free methods using highly sensitive microcapillary LC/MS/MS techniques at nanoliter flow rates. Several different types of mass spectrometry are utilized depending upon the needs of the researcher including a fast scanning and highly sensitive 2D linear ion trap and a high resolution/high mass accuracy LTQ-Oribitrap mass spectrometer acquired in 2007.
In 2005, in agreement with Harvard Medical School's Pathology Dept., the BIDMC Mass Spectrometry Core became part of the NRB Mass Spectrometry and Proteomics Core, expanding its services and became part of the Cancer Proteomics Core for the Dana Farber Harvard Cancer Center. We serve all institutions regardless of affiliation and have an interest in samples related to many diseases such as cancer and diabetes.
Recently, the core received much press attention for a Science article describing the use LC/MS/MS to sequence collagen protein fragments from a 68 million year old T. rex fossil.
We look forward to helping you progress your research with the most sensitive mass spectrometry technology that is currently available.
Personnel /Contact Information
Director: John M Asara, PhD
Phone: 617-735-2651
Fax: 617-735-2646
Email: jasara@bidmc.harvard.edu
Other Staff:
Xuemei Yang, M.S.
Research Associate
Services
- Protein (complex) Identification by LC/MS/MS
- Coomassie & silver-stained gel bands, solution samples after clean-up
- Mapping of Post-Translational Modifications by LC/MS/MS
- Coomassie-stained gel bands only, purified proteins (complexes) (phosphorylation, ubiquitinylation, acetylation, etc.)
- Relative Quantitation of Peptides by LC/MS/MS
- SILAC, iTRAQ, Isotope-Free Ratio Quantification
Special projects may be possible, please contact us to discuss your project needs.
Getting Started
More information regarding pricing, sample preparation and publications can be found at www.bidmcmassspec.org
