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Facilities and Equipment

Fluorescent Activated Cell Sorters


Beckman Coulter MoFlo: A high speed cell sorter (up to 50,000 cells/second) and analyzer (up to 70,000 cells/second), this instrument is a three laser system with a 35mW 635nm diode HeNe laser, a water cooled, 5W Coherent I-90C Krypton-Argon UV laser and a 200mW 488nm solid state diode pumped laser system. In addition to forward scatter and side scatter parameters, the instrument is equipped with eight interchangeable fluorescent parameters, allowing for five fluorescent parameters from the 488nm laser, two parameters from the UV laser and two parameters from the 635nm laser. Two- and Four- way sorting is available. Sorting can be done into tubes of various sizes, plates and custom slides. Single cell sorting into plates is also available.
Operating software for the MoFlo is the Beckman Coulter Summit program, version 4.3, running on a Dell Precision Intel Core 2 duo. Operation of this instrument requires a dedicated technician to align lasers, maintain the instrument and to assure its reliable and effective utilization.


Two Becton Dickinson SORP FACSAria IIs: These are special order five laser cell sorters (up to 22,000 cells/second) and analyzer (up to 70,000 cells/second) equipped with a solid state 50mW 488nm laser, JDS Uniphase 40mW 640nm HeNe laser, Coherent Compass 40mW 561nm laser (allowing for the acquisition, analysis and sorting of a variety of fluorescent proteins), Lightwave Mode Lock 60mW 355nm UV laser, and Coherent VioFlame 50mW 405nm violet laser. Besides the forward and side scatter parameters, the instrument is equipped for detection of four fluorescent parameters from the 488nm laser, three parameters from the 640nm laser, five parameters from the 561nm laser, three parameters from the violet laser and three parameters from the UV laser. Sorting can be done into tubes of various sizes, plates and custom slides. Single cell sorting into plates is also available.
Operating software for the FACSAria II is the Becton Dickinson Diva software, version 6.11, operating on an HP xw4600 Core 2 duo. Operation of this instrument requires a dedicated technician to maintain the instrument and to assure its reliable and effective utilization.

Beckman Coulter MoFlo XDP: A high speed cell sorter (up to 70,000 cells/second) and analyzer (up to 100,000 cells/second), this instrument is a four laser system with a 75mW 642nm diode laser, 355nm JDSU UV laser, Coherent VioFlame 50mW 405nm laser, and a 200mW CDHR 488nm solid state diode pumped laser system. In addition to forward scatter PMT and side scatter parameters, the instrument is equipped with eighteen interchangeable fluorescent parameters, allowing for six fluorescent parameters from the 488nm laser, six parameters from the 355nm laser or 405nm laser (run collinear) and six parameters from the 635nm laser. Two- and Four- way sorting is available. Sorting can be done into tubes of various sizes, plates and custom slides. Single cell sorting into plates is also available.
Operating software for the MoFlo is the Beckman Coulter Summit program, version 5.2, running on a Dell Precision Intel Core 2 duo. Operation of this instrument requires a dedicated technician to align lasers, maintain the instrument and to assure its reliable and effective utilization.

Flow Cytometry Analyzers


Becton Dickinson FACScan: This is a single laser, benchtop analyzer equipped with an air-cooled, argon laser (488nm), capable of detecting up to three fluorescent signals (FL1, FL2 and FL3), two physical signals (FSC and SSC) and two electronic signals (Area and Width). Software for acquisition and analysis is the Becton Dickinson CellQuest Pro, running on a Macintosh G5 computer. Individual research personnel, following requisite training from the Flow Cytometry Core staff, may independently use and operate this instrument. It is available to trained users for full use even in off hours, including evenings, nights, and weekends. Access to the instrument during off hours is controlled by a coded electronic card access system, which limits access to approved users only.

Beckman Coulter FC500: This is a dual laser, benchtop analyzer equipped with an air-cooled, 488nm laser and a JDS Uniphase 35mW 635nm laser. In addition to forward and side scatter parameters, the configuration of the dichroics allows for five fluorescent parameters off the 488nm laser or the combination of the 488nm and 635nm lasers (three fluorescent parameters from the 488nm laser and two parameters from the 635nm laser). Software for acquisition and analysis is the Beckman Coulter CXP, running on an Intel Pentium 4 processor. Individual research personnel, following requisite training from the Flow Cytometry Core staff, may independently use and operate this instrument. It is available to trained users for full use even in off hours, including evenings, nights, and weekends. Access to the instrument during off hours is controlled by a coded electronic card access system, which limits access to approved users only.

Becton Dickinson LSR II: This is a four laser, benchtop analyzer equipped with a solid state 488nm laser, JDS Uniphase 633nm HeNe laser, Lightwave Mode Lock 355nm UV laser, and Coherent VioFlame 405nm violet laser. Besides the forward and side scatter parameters, the instrument is equipped with five fluorescent parameters from the 488nm laser, two parameters from the 635nm laser, two parameters from the 405nm laser and two from the UV laser. Also, the instrument is equipped with an HTS (High Throughput sampler) for acquisition of 96, 384, and other customizable plate sizes. Operating software for the LSR II is the Becton Dickinson Diva software, version 6.11, operating on a HP xw4600 Core 2 duo. Individual research personnel, following requisite training from the Flow Cytometry Core staff, may independently use and operate this instrument. It is available to trained users for full use even in off hours, including evenings, nights, and weekends. Access to the instrument during off hours is controlled by a coded electronic card access system, which limits access to approved users only.

Becton Dickinson SORP LSR II: This is a special order five laser, benchtop analyzer equipped with a solid state 50mw 488nm laser, JDS Uniphase 40mW 640nm laser, a Coherent Compass 25mW 561nm laser (allowing for the acquisition and analysis of a variety of fluorescent proteins), a Coherent VioFlame 50mW 405nm laser, and a Mode-Lock 20mW 355nm laser. This allows for detection of four fluorescent parameters from the 488nm laser, three parameters from the 640nm laser, five parameters from the 561nm laser, three parameters from the violet laser and three parameters from the UV laser. Also, the instrument is equipped with an HTS (High Throughput sampler) for acquisition of 96, 384, and other customizable plate sizes. Operating software for the LSR II is the Becton Dickinson Diva software, version 6.11, operating on a HP xw4600 Core 2 duo. Individual research personnel, following requisite training from the Flow Cytometry Core staff, may independently use and operate this instrument. It is available to trained users for full use even in off hours, including evenings, nights, and weekends. Access to the instrument during off hours is controlled by a coded electronic card access system, which limits access to approved users only.

Beckman Coulter Gallios: The Gallios is a three laser benchtop analyzer equipped with a 22mW Argon 488nm laser, 25mW 638 laser, and a 40mW 405nm laser. All are solid state diodes which are spatially separated at 125um. Alignment is software controlled using Cyto System Mgmt software and can be controlled remotely. This allows for detection of five fluorescent parameters from the 488nm laser, three parameters from the 638nm laser, and two parameters from the 405nm laser in a proprietary "Boulevard" configuration for greater fluorescent resolution. With multiple measurements and angles of Forward Scatter and a gel coupled pickup filter lens assembly, the Gallios can resolve 0.4um diameter particles from background and maximize detection up to 40um. This instrument is equipped with a Multi-tube Carousel Loader system to automatically analyze multiple samples. Software for acquisition and analysis is the Beckman Coulter CXP, running on an Intel Pentium Core 2 Duo processor. Individual research personnel, following requisite training from the Flow Cytometry Core staff, may independently use and operate this instrument. It is available to trained users for full use even in off hours, including evenings, nights, and weekends. Access to the instrument during off hours is controlled by a coded electronic card access system, which limits access to approved users only.

Cyntellect Celigo: The Celigo adherent cell cytometer allows in situ brightfield and fluorescent analysis of adherent cells. Cell number, density, area density, diameter, area, and perimeter can all be measured. Using LEDs, the system can obtain data for three fluorescent channels (ex 377nm/em 447nm, ex 483nm/em 536nm, and ex 531nm/em 629nm) and 1 brightfield channel. 384, 96, 24, 12, 6, and 1536 well plates can be analyzed; as well as, T-25 and T-75 flasks. Software for acquisition and analysis is the Cyntellect Celigo software version 1.3, running on a Dell Precision T3500. Individual research personnel, following requisite training from the Flow Cytometry Core staff, may independently use and operate this instrument. It is available to trained users for full use even in off hours, including evenings, nights, and weekends. Access to the instrument during off hours is controlled by a coded electronic card access system, which limits access to approved users only.

Miltenyi AutoMACS Magnetic Separator: A self-run bench-top instrument for high-speed automated cell separation. Gentle isolation of viable, functionally active cells resulting in excellent purity and recovery of rare cells. Flexible cell sorting strategies: positive selections, depletions, or sequential sorting. On instrument, touch screen control allows for ease of use.

• Ampligrid and Amplispeed: Beckman Coulter is introducing a superior amplification platform for ultra low volume applications in the 1µL range, based on a chemically structured microscope slide. With this innovative system, sensitivity, efficiency and reproducibility are reliable constants for single cell analysis, low-copy number approaches and ultra low volume amplification techniques. And as a result fast and convenient workflow is assured. The AmpliGrid system is the key to obtaining perfect results especially with single cells and nearly any other kind of template material e.g., forensic stains or purified nucleic acids. The AmpliSpeed slide cycler is an ultra-compact instrument specifically designed for performing thermal cycling reactions on glass slides. An optimal thermal coupling with the glass slides is guaranteed using a superior heat transfer technology based on highly-planar silicon wafers. This unique system generates extremely homogenous surface temperatures with effective heating and cooling rates of up to 3°C/sec. This can definitely be classified as HIGH-SPEED.

Analysis Software


FlowJo: FlowJo software reads flow cytometry data and facilitates complex data analysis in a graphically intuitive way.

Beckman Coulter Kaluza: Kaluza Software challenges today´s solutions with a new vision of what flow cytometry analysis should be. Kaluza provides visualization tools, speed and an innovative simplicity to the flow community. With features designed to make your life easier in the lab, Kaluza aims to provide a greater understanding of the data generated by modern cytometers. Kaluza employs patent pending technology that allows for real time analysis of high content files allowing you to spend your valuable time on discovery.

Training


• Training is available for all Flow Cytometry Analyzers and the FACSAria II Cell Sorter. Trainings are provided by an experienced staff member several times monthly. Also, a "Basics of Flow Cytometry" course is offered 1-2 times per year based on request. In addition, researchers can request Facility run or Facility assisted analysis of samples.

• Educational seminars and workshops are provided several times per year for the advancement of the research community.


Contact Information

Flow Cytometry Core
Beth Israel Deaconess Medical Center
Center for Life Science, CLS-0932
330 Brookline Ave
Boston, MA 02215
617-735-4191
617-735-4190