beth israel deaconess medical center a harvard medical school teaching hospital

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Zeiss LSM510 Upright Confocal System


Meta Detector
Allows for spectral imaging and
online emission fingerprinting.

Zeiss Axioimager Z1 microscope (upright)

10x Zeiss EC Plan-Neofluar dry, 0.3 NA
20x Zeiss Apochromat dry, 0.8 NA
40x Zeiss Apochromat Oil, 1.3 NA, DIC (UV) VIS IR
63X Zeiss Apochromat Oil, 1.4 NA, DIC, IR
100X Zeiss Apochromat Oil, 1.4 NA, DIC, IR

Fluorescence Filters Cubes (for visual inspection)
DAPI: 365 nm excitation, 445/50 nm emission.
FITC / GFP: 470/40 nm excitation, 525/50 nm emission.
Rhodamine: BP 545/25 nm excitation, 605/70 nm emission.
CY5: BP 640/30 nm excitation, BP 690/50 nm emission.

Laser Lines (for scanning)
405 nm: solid-state laser, 25 mW
458, 488 and 514 nm: Argon ion laser, 30 mW
543 nm: Helium-Neon laser, 1mW
633 nm: Helium-Neon laser, 15mW

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Contact Information

Confocal Imaging Core
Beth Israel Deaconess Medical Center
Dana 814
330 Brookline Avenue
Boston, MA 02215