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Zebrafish Core

Summary:

The BIDMC Zebrafish Core Facility is is a newly designed 3,000+ fish tank facility, located in the Slosberg-Landay building. It is a state-of-the-art 2,500 sqft facility built by Pentair Aquatic Habitats. The facility is equiped with 48 racks which hold standard sized adult zebrafish tanks, a three rack nursery space for larval fish, and a quarantine space containing two stand-alone housing systems. Wild-type lines are maintained inhouse for shared use. Wet lab space is available for breeding and line maintenance; core staff and facility-associated technicians perform daily feeding. The core includes a dedicated procedure room for embryo manipulation. Since 2011, a full time manager staffs the facility and is available for assistance with access, training and general Zebrafish husbandy questions. All BIDMC Zebrafish Core users must be associated with an IACUC-approved animal protocol.

Services:

  • Housing and general care of Zebrafish (feeding, health monitoring, system maintenance)
  • Propagation of wild-type Zebrafish stocks for embryo production
  • Training in Zebrafish handling and husbandry
  • Assistance with Zebrafish protocol planning and preparation
  • Shipping and receiving live Zebrafish

Equipment:

  • 3000 tank Aquatic Habitats recirculating water system (1, 5, 10L tanks), including nursery area
  • 2 stand-alone isolation/quarantine units
  • 4 Labconco steam scrubber dishwashers
  • 4 Narishige microinjectors
  • 4 Leica steromicroscopes
  • 1 Vevo800 high-frequency ultrasound machine
  • 2 Variable temperature incubators
  • 4 Vibration-stabilized microinjection stations
  • 3 YSI 5200 water quality monitors
  • 3 PT4 TGP monitors

Access and Use:

ALL users must…

  • Be listed on an IACUC-approved animal protocol (for Zebrafish)
  • Under go training on BIDMC Core Facility rules and standard operations prior to gaining card access
  • Perform BIDMC ARF and CITI Training as appropriate to Zebrafish use
  • Have BIDMC Health Clearances to work with animals
  • Work humanely with experimental Zebrafish lines, following IACUC approved standard procedures for housing densities and tank maintenance (see SOPs)
  • Participate in weekend/holiday feeding coverage rotation

Fee Structure:

Rack Fee (single bookcase of tanks in any configuration and population density):

  • BIDMC Internal Rate: $125.00 per rack monthly
  • External Special Rate: $125 per rack monthly
  • External Academic: $180.00 per rack monthly

Nursery/Quarantine fee: $50.00 flat rate monthly for use
Technician Fee*: $30.00 flat fee monthly (*waved if facility provided technical support)

Contact:

Core Director
Trista E North, PhD
Tel:  617-735-2083
Email:  tnorth@bidmc.harvard.edu 

Core Coordinator
Michelle Vedder
Tel:  617-667-8943
Email:  mvedder@bidmc.harvard.edu

 

Contact Information

BIDMC Zebrafish Core
Director: Dr. Trista E North
Slosberg-Landay Building
330 Brookline Ave.
Boston, MA 02215
Tel: 617-735-2083
Email: tnorth@bidmc.harvard.edu